The activated sludge cultured by surfactant domestication was used as the biodegradation source, added to the test portion for shaking culture, and the decrease of the surfactant during the culture period was measured to calculate the biodegradability of the sample. The complex formed by the nonionic surfactant and cobalamin thiocyanate is extracted with benzene, and then the nonionic surfactant is quantified by spectrophotometry.
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